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Polymerase Chain Reaction (PCR): DNA Amplification
PCR is based on the mechanisms of DNA replication. First, the double-stranded DNA, which serves as the template in the reaction, is separated by heat. Next, a DNA polymerase synthesizes a new daughter strand on each single strand in one direction. By repeating these reactions continuously, a defined segment of DNA, the target, is amplified ...
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En una PCR, los primers son pequeños fragmentos de DNA que funcionan como puntos de inicio para que la enzima copie una región específica del material genético. En otras palabras, le dicen a la reacción exactamente qué segmento quieres amplificar. Diseñarlos bien es clave, porque si haces unos primers “chafas”, la reacción puede amplificar cosas que no quieres, no amplificar nada, o generar resultados confusos. Un buen diseño considera aspectos como la longitud, la temperatura de alineamiento, e
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