The infected mosquitoes have been located in several city neighborhoods, including Old Louisville, Irish Hill, California, Iroquois and Churchill Downs, officials said July 22. This follows mosquitoes testing positive for the virus in the Tyler Park and Highlands neighborhoods earlier this month.

A southern Indiana resident has Indiana’s first case of West Nile virus for the year, the Indiana Department of Health announced Tuesday.

There have been three human cases of West Nile Virus in California this year, with one fatal case, according to the California Department of Public Health.