Real-time quantitative PCR (RT-qPCR or qPCR) is revolutionizing many aspects of molecular biology and becoming the gold standard for accurate, sensitive, and fast quantification of gene expression.

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Researchers have built an RT-PCR platform that gives results in 23 minutes that match the longer laboratory-based tests -- faster than other PCR tests on the market. It can be adapted to test for a ...

Respiratory syncytial virus (RSV) was detected easiest when using reverse transcription polymerase chain reaction (RT-PCR) tests in a pediatric population. Reverse transcription polymerase chain ...

In a recent study published in PLOS ONE, researchers developed a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) system to identify severe acute respiratory syndrome coronavirus ...

Correlation of HER2 low status in I-SPY2 with molecular subtype, response, and survival.

Real-time PCR plays a foundational role in gene expression studies, disease analysis, and molecular diagnostics. However, success depends on precise reaction setup, optimized assays, and accurate ...